We provide high quality isolation of both DNA and RNA as an additional service to our metagenomic assays. Having completed more than 300 microbiome studies, our team has optimized its isolation protocols to isolate RNA and DNA that remains representative of the microbial community being sampled. Different sample types have different extraction requirements and protocols must be specialized to ensure the isolation procedure does not alter the microbiome profile.
Second Genome Solutions offers DNA isolation in conjunction with our end-to-end services. Our DNA isolation services are highly scalable and can support large study sample sets. With the ability to isolate DNA from more than 25 different sample types, including human and mouse fecal specimens, surface swabs, needle aspirates and lavage fluid, we are experts in obtaining high yield and integrity. We have dedicated resources for isolating low biomass specimens.
DNA extraction or DNA isolation is the process by which DNA is removed and collected from a given sample and then used in subsequent diagnostic processes to, for example, detect and identify cells in the environment and conduct genetic studies. DNA extraction usually employs both physical and chemical methods to remove non-DNA material and is a standard method in molecular biology and forensics.
We also offer extraction of highly pure and intact total RNA from a variety of samples including stool, tissue, saliva, plants, and other environmental samples. Concomitant extraction of genomic DNA is possible if sufficient sample material is available to conduct both isolations.
Below is a subset of specimens from which we have successfully isolation microbial genetic material:
- Bronchial lavage
- Oral bioflims
- Plant (leaf, root, rhizosphere)